WES Analysis Report

MegaBOLT v2.4.0.0_release

1.Base summary

QC result

Sample Sample
Base_raw 11,291,464,704
Read_length 150:150
Read_raw 75,276,432
Read_clean 74,509,504
Read_clean_Rate 98.98%
Q20_raw 95.14%
Q20_1_raw 95.87%
Q20_2_raw 94.42%
Q30_raw 86.31%
Q30_1_raw 87.84%
Q30_2_raw 84.79%
GC_raw 43.48%
Q20_clean 95.18%
Q20_1_clean 95.93%
Q20_2_clean 94.42%
Q30_clean 86.34%
Q30_1_clean 87.89%
Q30_2_clean 84.8%
GC_clean 43.54%

Base distribution

Base quality distribution

2.Alignment Information

Alignment result

Sample Sample
Mapping_Rate 99.29%
PE_Mapping_Rate 98.74%
Unique_Rate 93.02%
Duplication_Rate 5.63%
Mismatch_Rate 0.92%
Chimerical_Rate 1.67%
Insert_size 302.7
Capture_Rate_on_Reads 75.41%
Capture_Rate_on_Bases 66.31%
Average_depth(rmdup) 100.76
Coverage(>=1X) 99.01%
Coverage(>=4X) 98.17%
Coverage(>=10X) 96.68%
Coverage(>=20X) 92.39%
Coverage(>=30X) 85.12%
Coverage(>=100X) 34.29%
Uniformity(>0.2f) 91.78%
FOLD_80_BASE_PENALTY 2.879

Insert size distribution

Depth distribution

3.Variant calling

Variant statistics

Sample Sample
Total_SNP 65,806
dbSNP_Rate 98.92%
Novel_SNP 712
Novel_SNP_Rate 1.08%
Ti/Tv 2.48
Total_InDel 7,313
dbInDel_Rate 68.58%

4.Variant evaluation

SNP evaluation

Threshold True-pos-baseline True-pos-call False-pos False-neg Precision Sensitivity F-measure
6 54,670 54,670 75 1,127 0.9986 0.9798 0.9891
None 54,671 54,671 78 1,126 0.9986 0.9798 0.9891

InDel evaluation

Threshold True-pos-baseline True-pos-call False-pos False-neg Precision Sensitivity F-measure
8 4,486 4,486 384 741 0.9211 0.8582 0.8886
None 4,497 4,497 407 730 0.9170 0.8603 0.8878