WES Analysis Report

MegaBOLT v3.0.0.0_release

1.Base summary

QC result

Sample V5
Raw base number 11,291,465,100
Read length 150:150
Raw read number 75,276,434
Clean read number 74,433,498
Clean Read rate 98.88%
Raw read Q20 95.14%
Clean read Q20 95.18%
Raw read1 Q20 95.87%
Clean read1 Q20 95.93%
Raw read2 Q20 94.42%
Clean read2 Q20 94.42%
Raw read Q30 86.31%
Clean read Q30 86.34%
Raw read1 Q30 87.84%
Clean read1 Q30 87.89%
Raw read2 Q30 84.79%
Clean read2 Q30 84.79%
Raw read GC rate 43.48%
Clean read GC rate 43.54%

Base distribution

Base quality distribution

2.Alignment Information

Alignment result

Sample V5
Mapping Rate 99.29%
PE Mapping Rate 98.75%
Unique Rate 93.02%
Duplication Rate 5.64%
Mismatch Rate 0.92%
Chimerical Rate 1.64%
Insert size 302.9
Capture Rate on Reads 75.41%
Capture Rate on Bases 66.31%
Average depth(rmdup) 100.63
Coverage(>=1X) 99.01%
Coverage(>=4X) 98.17%
Coverage(>=10X) 96.67%
Coverage(>=20X) 92.37%
Coverage(>=30X) 85.08%
Coverage(>=100X) 34.23%
Uniformity(>0.2f) 91.77%
FOLD_80_BASE_PENALTY 2.877

Insert size distribution

Depth distribution

3.Variant calling

Variant statistics

Sample V5
Total SNP number 65,776
dbSNP Rate 98.93%
Novel SNP number 702
Novel SNP Rate 1.07%
Ti/Tv 2.48
Total InDel number 7,315
dbInDel Rate 68.56%