WGS Analysis Report

MegaBOLT v3.0.0.0_release

1.Base summary

QC result

Sample PCR-free-1-PE150-1
Raw base number 126,174,119,100
Read length 150:150
Raw read number 841,160,794
Clean read number 838,959,490
Clean Read rate 99.74%
Raw read Q20 96%
Clean read Q20 96%
Raw read1 Q20 96.07%
Clean read1 Q20 96.07%
Raw read2 Q20 95.93%
Clean read2 Q20 95.93%
Raw read Q30 86.76%
Clean read Q30 86.75%
Raw read1 Q30 87.4%
Clean read1 Q30 87.39%
Raw read2 Q30 86.12%
Clean read2 Q30 86.11%
Raw read GC rate 41.07%
Clean read GC rate 41.07%

Base distribution

Base quality distribution

2.Alignment Information

Alignment result

Sample PCR-free-1-PE150-1
Mapping Rate 99.95%
PE Mapping Rate 99.92%
Unique Rate 96.3%
Duplication Rate 0.97%
Mismatch Rate 0.75%
Insert size 404.5
Average depth(rmdup) 42.8
Coverage(>=1X) 99.09%
Coverage(>=4X) 98.88%
Coverage(>=10X) 98.48%
Coverage(>=20X) 97.03%
Coverage(>=30X) 89.50%
Coverage(>=100X) 0.32%
Uniformity(>0.2f) 98.56%

Insert size distribution

Depth distribution

3.Variant calling

Variant statistics

Sample PCR-free-1-PE150-1
Total SNP number 3,962,925
dbSNP Rate 94.07%
Novel SNP number 234,820
Novel SNP Rate 5.93%
Ti/Tv 1.97
Total InDel number 946,118
dbInDel Rate 77.4%