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Whole-Genome Sequencing Facilitates CRISPR-Cas12f1 System Upgrade
The gRNA within CRISPR/Cas has been found to play a key role in reducing off-target effects. Therefore, the redesign and remodeling of gRNAs could help increase the capabilities of CRISPR tools in terms of simplicity, multiplexing, and specificity. To optimize the Cas12f1 gRNA, Do Yon Kim and colleagues identified five potential modification sites (MSs) in the tracrRNA and crRNA sequences for remodeling to produce a powerful and compact CRISPR-Cas12f1 system. Based on data powered by MGI’s ult...
More2022-05-17