Unleash Your Sequencing Speed
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Unleash Your Sequencing Speed

As one of the sequencers with the fastest speed among mid-low throughput in the world, DNBSEQ-G99(1) is developed based on MGI's core DNBSEQTM sequencing technology, enabled by innovations in biochemistry, optics, fluidics, temperature control, and other core systems. DNBSEQ-G99 is especially applicable for multiple sequencing methods, it only needs 12 hours to finish PE150 sequencing with Q40 data.

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Features

Features



8 Gb~96 Gb per run                                


Only 12 hrs for PE150 (from loading to FASTQ)                                       

Supports multiple read lengths, including PE50, SE100, PE150, PE300, SE400                             
Applicable to multiple sequencing methods

Performance Parameter

Maximum number of Flow cells  

Lanes/Flow cell

Effective Reads*/Flow Cell

Supported Reads Lengths** Data Output Q30***

Q40***

Run Time****


2


1


80 M

SE100/PE50

8 Gb~16 Gb >90% >85% 5 h

PE150

24 Gb~48 Gb

>85% >85% 12 h

PE300

48 Gb~96 Gb

>85% >80% 30 h

SE400

32 Gb~64 Gb

>75% >70%
20 h
* The effective reads are based on the sequencing of an internal standard library. Actual output may vary depending on sample type and library preparation method.
** DNBSEQ-G99 also supports SE50 and PE100 sequencing, and the existing kits can support SE50, PE100 read length sequencing.
*** The percentage of bases above Q30 is the average of an internal standard library over the entire run. Actual performance is affected by factors such as sample type, library quality, and insert fragment length. Only StandardMPS 2.0(SM 2.0) reagents support the generation of Q40 data.
**** The sequencing time is the statistical duration for single flow cell sequencing and dual flow cell simultaneous sequencing.
Method Application

Recommended read length

Data size Sample per Run

1 flow cell

2 flow cells





Targeted Capture/

Multiplex PCR

Oncology panel

PE100/ PE150

Small panel: ~1 Gb/Sample

24 48
Genetic disease diagnosis (small panel) PE150

Deafiness: ~5 Gb/Sample

Thalassemia: ~0.2 M reads/Sample

4

400

8

800

ATOPlex panel

PE100/ PE150

Respiratory tract panel: 5 M reads/Sample

SARS-CoV-2 panel: 5 M reads/Sample

16 32
WES PE150 15 Gb/Sample 1~2 2~4
Methylation
Oncology targeted methylation panel PE150 ~5 Gb/Sample 4 8


Small Genome Sequencing

Metagenomics for pathogen detection SE50/ SE100 Meta: 20 M reads/Sampple 4 8
Microbial WGS PE100/ PE150 Single bacterium: ~1 Gb/Sample 16~24 32~48
16S Metagenomic Sequencing
PE300 ≥0.1 M reads /Sample
576 1152

LOW pass whole-genome 

sequencing

NIPT SE50


~10 M reads/Sample


8


16

PGS SE50

Transcriptome sequencing


RNA-Seq SE50/ PE150

Expression profiling: ~25 M reads/Sample

Transcriptome: ~6 Gb/Sample

3

4

6

8

Frensics
DNA signature identification
SE400 ~0.8 M reads/sample
96 192
*Methods indicated with are the key applications of DNBSEQ-G99.
** Recommended data output and sample numbers are only for reference, actual application will require optimisation adjustments.


Cat. No Product Name
900-000607-00 DNBSEQ-G99RS
900-000609-00 DNBSEQ-G99ARS
940-001268-00
DNBSEQ-G99RS High-throughput Sequencing Reagent Set (G99 FCL SE100/PE50)
940-001269-00
DNBSEQ-G99RS High-throughput Sequencing Reagent Set (G99 FCL PE150)
940-001267-00
DNBSEQ-G99RS High-throughput Sequencing Reagent Set (G99 App-D FCL SE100)
940-001274-00
DNBSEQ-G99RS High-throughput Sequencing Reagent Set (G99 App-D FCL PE150)
940-001716-00

DNBSEQ-G99RS High-throughput Sequencing Reagent Set (G99 App-D FCL PE300)

940-001757-00
DNBSEQ-G99RS High-throughput Sequencing Reagent Set (G99 FCL SE400)
940-000409-00 High-throughput Sequencing Set (G99 SM FCL SE100/PE50)
940-000410-00 High-throughput Sequencing Set (G99 SM FCL PE150)
940-000520-00
High-throughput Sequencing Set (G99 SM App-C FCL SE100)
940-000413-00
High-throughput Sequencing Set (G99 SM App-C FCL PE150)
940-000415-00
High-throughput Sequencing Set (G99 SM FCL PE300)
940-000417-00
High-throughput Sequencing Set (G99 SM FCL SE400)
940-000624-00 DNBSEQ-G99 Cleaning Reagent Kit
Selected as needed UPS


System Parameter

  • Product Model

    DNBSEQ-G99 (Without Bioinformatics Module)

    DNBSEQ-G99A (With Bioinformatics Module)

    DNBSEQ-G99RS (Without Bioinformatics Module)(1)

    DNBSEQ-G99ARS (With Bioinformatics Module)(1)


    (1) For research use only. Not for use in diagnostic procedures.


(1) Unless otherwise informed, this StandardMPS sequencing reagent is not available in Germany, UK, Sweden, and Switzerland.
  • Hotline (Global)
    +86-4000-688-114
  • Email
    MGI-service@mgi-tech.com
  • Special Recommendations
  • *For research use only.

    Not for use in diagnostic procedures (except as specifically noted).
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