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ATOPlex RNA Library Prep Set

ATOPlex RNA Library Prep Set includes RT and two-steps PCR that convert viral RNA to sequence libraries in 5 hours. With unique technology, ATOPlex performs high specificity of amplification and low dimer rate. Moreover, the ATOPlex kit includes clean system and spike-in system which minimizes cross-contamination risk and improves the accuracy of virus loads quantification. It is suitable for population-scale virus detection, surveillance, and tracing.


ATOPlex RNA Library Prep Set

Item No.:940-000183-00

RT and PCR Reagment

ATOPlex RNA Universal Library Preparation Module

940-000181-00

Primer of SARS-CoV-2

Internal Control Primer Pool
940-000241-00

Dual Barcode Primer

ATOPlex Dual Barcode Primer Module (01-96)

1000021626

Dual Barcode Balance Library

ATOPlex Dual Barcoded Balance Library Reagent

1000022270

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Features

Ultra-sensitive Detection
Ultra-sensitive Detection

Ultra-sensitive Detection with as Low as 10 Copies/mL Viral Loads


Full-length Genome Coverage
Full-length Genome Coverage

>99% Full-length Genome Coverage with Challenge Sample


Simple operation
Simple operation
Easy Protocol with RT and Two-step PCR in One Tube
Accurate quantification
Accurate quantification
Accurate Quantification by Spike-in Control
Ultra-sensitive Detection
Ultra-sensitive Detection

Ultra-sensitive Detection with as Low as 10 Copies/mL Viral Loads


Full-length Genome Coverage
Full-length Genome Coverage

>99% Full-length Genome Coverage with Challenge Sample


Simple operation
Simple operation
Easy Protocol with RT and Two-step PCR in One Tube
Accurate quantification
Accurate quantification
Accurate Quantification by Spike-in Control

Product Specification

Product Name

ATOPlex RNA Library Prep Set

Configuration 96 Preps/kit
Sample Types

Total Nucleic Acid from Nasopharyngeal Swabs, BALF, etc

Application 

Surveillance, Variation and Evolution Analysis of SARS-CoV-2

Detection Region
SARS-CoV-2 Full Length
Amplicons Size 106-199 bps
Amplicons Numbles
273 Amplicons in one tube
cDNA input

>10 copies genome for full length,>10 copies/mL for detection

Variant Types

SNP,InDel

Total Time(sample to library)
5 hrs
Uniformity(0.1X)
95%
On Target Aligned Reads
≥95%
Coverage
≥99%
Recommend Sequence Type
PE100 for Full Length Genome,SE50 for Detection
Recommend Total Reads
0.5-3 M Reads/sample

Recommended MGI Process

Applications
Library preparation
Sequencing
Bioinformation analysis
  • Surveillance, Variation and Evolution Analysis of SARS-CoV-2

  • ATOPlex RNA Library Prep Set


  • Fast identification of pathogenic infections(PFI) and MGAP(Microbial Genome Analysis Pipeline)
  • MGI-SARS-CoV-2 pipeline

  • ATOPlex SARS-CoV-2 software

*Remark: *Unless otherwise informed, StandardMPS and CoolMPS sequencing reagents, and sequencers for use with such reagents are not available in Germany, USA, Spain, UK, Hong Kong, Sweden, Belgium, Italy, Finland, Czech Republic, Switzerland and Portugal.

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