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DNBSEQ-G50RS High-throughput (Rapid) Sequencing Set User Manual

DNBSEQ-G50RS High-throughput Sequencing Set and DNBSEQ-G50RS High-throughput Rapid Sequencing Set are specifically designed for DNA or RNA sequencing on DNBSEQ-G50RS. This sequencing set is intended to be used for scientific researches only and cannot be used for clinical diagnoses.

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DNBSEQ-G50RS High-throughput (Rapid) Sequencing Set User Manual

DNBSEQ-G50RS High-throughput Sequencing Set (FCL SE50)*

Item No.：1000019855

1000019849	DNBSEQ-G50RS High-throughput Sequencing Kit (FCL SE50/FCS SE100)
1000020208	DNBSEQ-G50RS Sequencing Flow Cell

DNBSEQ-G50RS High-throughput Sequencing Set (FCL SE100)*

Item No.：1000019856

1000019850	DNBSEQ-G50RS High-throughput Sequencing Kit (FCL SE100)
1000020208	DNBSEQ-G50RS Sequencing Flow Cell

DNBSEQ-G50RS High-throughput Sequencing Set (FCL PE50)*

Item No.：1000019857

1000019851	DNBSEQ-G50RS High-throughput Sequencing Kit (FCL PE50/FCS PE100)
1000020208	DNBSEQ-G50RS Sequencing Flow Cell

DNBSEQ-G50RS High-throughput Sequencing Set (FCL PE100)*

Item No.：1000019859

1000019853	DNBSEQ-G50RS High-throughput Sequencing Kit (FCL PE100/FCS PE150)
1000020208	DNBSEQ-G50RS Sequencing Flow Cell

DNBSEQ-G50RS High-throughput Sequencing Set (FCL PE150)*

Item No.：1000019858

1000019852	DNBSEQ-G50RS High-throughput Sequencing Kit (FCL PE150)
1000020208	DNBSEQ-G50RS Sequencing Flow Cell

DNBSEQ-G50RS High-throughput Rapid Sequencing Set (FCS SE100)*

Item No.：1000019860

1000019849	DNBSEQ-G50RS High-throughput Sequencing Kit (FCL SE50/FCS SE100)
1000020209	DNBSEQ-G50RS Rapid Sequencing Flow Cell

DNBSEQ-G50RS High-throughput Rapid Sequencing Set (FCS PE100)*

Item No.：1000019861

1000019851	DNBSEQ-G50RS High-throughput Sequencing Kit (FCL PE50/FCS PE100)
1000020209	DNBSEQ-G50RS Rapid Sequencing Flow Cell

DNBSEQ-G50RS High-throughput Rapid Sequencing Set (FCS PE150)*

Item No.：1000019862

1000019853	DNBSEQ-G50RS High-throughput Sequencing Kit (FCL PE100/FCS PE150)
1000020209	DNBSEQ-G50RS Rapid Sequencing Flow Cell

Features

More flexible 10～150 Gb per run	Shorter turnaround time Optimization in sequencing chemistry, FCS flow cell SE100 sequencing in 10 hours
Longer read length Release of PE150, enabling more applications	Better quality Optimized sequencing chemistry and base calling algorithm, improving paired-end data quality with more accurate base calling

More flexible

10～150 Gb per run

Shorter turnaround time

Optimization in sequencing chemistry, FCS flow cell SE100 sequencing in 10 hours

Longer read length

Release of PE150, enabling more applications

Better quality

Optimized sequencing chemistry and base calling algorithm, improving paired-end data quality with more accurate base calling

Product Specification

Read length	SE50-FCL	SE100-FCL	PE50-FCL	PE100-FCL
Maximum cycles	70	120	120	220
Effective reads/flow cell*	500M	500M	500M	500M
Data output/flow cell	25Gb	50Gb	50Gb	100Gb
Q30*	>85%	>85%	>85%	>85%
Run time**	～11hrs	～17hrs	～24hrs	～47hrs

Read length	PE150-FCL	SE100-FCS	PE100-FCS	PE150-FCS
Maximum cycles	320	120	220	320
Effective reads/flow cell*	500M	100M	100M	75M
Data output/flow cell	150Gb	10Gb	20Gb	22.5Gb
Q30*	>80%	>80%	>85%	>80%
Run time**	～66hrs	～10hrs	～30hrs	～43hrs
* Effective reads and Q30 are based on the standard library. These values may vary with various applications. **Run time includes DNB loading, sequencing, and data process (Fastq writing). Run time may vary with various instruments.

*Remark:*Unless otherwise informed, StandardMPS and CoolMPS sequencing reagents, and sequencers for use with such reagents are not available in Germany, USA, Spain, UK, Hong Kong, Sweden, Belgium, Italy, Finland, Czech Republic, Switzerland and Portugal.