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MGIEasy Exome Universal Library Prep Set

MGIEasy Exome Universal Library Prep Set aims to prepare libraries with human gDNA. DNA is fragmented by Covaris, followed by the steps of adaptor ligation at both ends and PCR amplification. The amplified PCR product can be used for exome capture with MGIEasy Exome Capture V4/V5 Probe Set or other commercialized probes.

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MGIEasy Exome Universal Library Prep Set V1.0 (16 RXN)

Item No.：1000009657

Core library preparation kit	MGIEasy Universal DNA Library Prep Kit	1000005248
Adaptors	MGIEasy DNA Adapters-16 (Tube) kit V1.0	1000005284
Clean beads	MGIEasy DNA Clean Beads	1000005278
Circularization	MGIEasy Circularization Kit V2.0	1000005260
Other	MGIEasy Exome Capture Accessory Kit	1000007743

Features

Compatible with exome probes from various companies The constructed libraries are compatible with probes from Agilent, Nimblegen, IDT,and etc.	Compatible with the automation systems Multiple steps can be finished with the automation systems MGISP-100, such as steps from fragmented DNA to pre-PCR, as well as post-capture elution to post-PCR.
Quick to prepare libraries and easy to operate Library preparation time 7h (without hybridization capture time), hands-on time ~30min.

Compatible with exome probes from various companies

The constructed libraries are compatible with probes from Agilent, Nimblegen, IDT,and etc.

Compatible with the automation systems

Multiple steps can be finished with the automation systems MGISP-100, such as steps from fragmented DNA to pre-PCR, as well as post-capture elution to post-PCR.

Quick to prepare libraries and easy to operate

Library preparation time 7h (without hybridization capture time), hands-on time ~30min.

Recommended Applications

Library preparation set	Probes for capture	Compatible sequencing	Read length	Applications
MGIEasy Exome Universal Library Prep Set	MGIEasy Exome Capture V4 Probe Set	DNBSEQ-T7 DNBSEQ-G400 DNBSEQ-G50	PE100/PE150	Whole exome sequencing
MGIEasy Exome Capture V5 Probe Set	PE100/PE150	Whole exome sequencing
Other commercialized probes	PE100/PE150	Whole exome sequencing/ target sequencing

Library preparation set

Probes for capture

Compatible sequencing

Read length

Applications

MGIEasy Exome Universal Library Prep Set

MGIEasy Exome Capture V4 Probe Set

DNBSEQ-T7

DNBSEQ-G400

DNBSEQ-G50

PE100/PE150

Whole exome sequencing

MGIEasy Exome Capture V5 Probe Set

PE100/PE150

Whole exome sequencing

Other commercialized probes

PE100/PE150

Whole exome sequencing/ target sequencing

Product Specification

Product name	MGIEasy Exome Universal Library Prep Set
Version	V2.0
Packaging (Part number)	16 RXN （1000009657）
Storage	-25℃~-18℃, 2℃~8℃（Refer to kit label）
Required sample input	10 ng -1000 ng fragmented gDNA; DNA concentration: ≥12.5 ng/μL; intact or mildly degraded DNA; D260/280=1.8~2.0
Sample types	Tissues, blood, FFPE samples, cfDNA
Compatible species	Human
Library preparation time	~7 h (without hybridization capture time)
Hands-on time	~30 mins
Compatible probes	Probes MGI, Agilent, Nimblegen, IDT
Fragmentation methods	Covaris (no fragmentation for cfDNA)
Library fragment size	200-600 bp
Compatible sequencing platforms	DNBSEQ-T7, DNBSEQ-G400, DNBSEQ-G50
Read length	PE100/PE150

Recommended MGI Process

Applications	Library preparation	Sequencing	Bioinformation analysis
Whole exome sequencing	MGISP-100 MGIEasy Exome Universal Library Prep Set MGIEasy Exome Capture V4/V5 Probe Set, other commercialized probes	DNBSEQ-G400, DNBSEQ-T7 (PE100, PE150)

Applications

Library preparation

Sequencing

Bioinformation analysis

Whole exome sequencing

MGISP-100
MGIEasy Exome Universal Library Prep Set
MGIEasy Exome Capture V4/V5 Probe Set, other commercialized probes

DNBSEQ-G400*, DNBSEQ-T7* (PE100, PE150)

Related Products

MGIEasy Exome Capture V4 Probe Set

Applications: To capture the exome of human genome. It also covers more than 99% disease-related regions.

Suitable sequencing platforms: DNBSEQ-G400, DNBSEQ-T7

Reaction/kit: 16RXN/kit

MGIEasy Exome Capture V5 Probe Set

Applications: To capture the exome of human genome. It also ensures the comprehensive capture of coding sequences related to various diseases by targeted design, e.g. reproductive, neonatal, cardiovascular and cerebrovascular, hereditary tumors/deafness, monogenic, medication safety, personal genome, immunodeficiency, mitochondrial defects, etc.

Suitable sequencing platforms: DNBSEQ-G400, DNBSEQ-T7

Reaction/kit: 16 RXN/kit

MGIEasy Exome Universal Library Prep Set