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MGIEasy Exome Capture V4 Probe Set

MGIEasy Exome Capture V4 Probe Set is the first exome capture product that is independently designed by MGI. The databases used for probe design include CCDS, GENCODE, RefSeq, miRBase and so on. The probe set can be flexibly matched with MGIEasy Exome Universal Library Prep Set or MGIEasy Exome FS Library Prep Set for the construction and hybridization of libraries for MGI sequencing platforms; it can also be matched with other library preparation kits for other high throughput sequencing platforms.

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MGIEasy Exome Capture V4 Probe Set（16 RXN）

Item No.：940-000186-00

Probes

MGIEasy Exome Capture V4 Probe（16RXN）

1000007740

Hybridization and elution

MGIEasy Exome Capture Hybridization and Wash Kit (Box 1) （16RXN）

940-000168-00

Hybridization and elution

MGIEasy Exome Capture Hybridization and Wash Kit (Box 2) （16RXN）

940-000169-00

Features

Probe regions 59Mb, covering over 20000（refGene）genes The databases used for probe design include CCDS、GENCODE、RefSeq、miRBase and so on.	Deep and comprehensive coverage In HGMD and ClinVar databases, MGIEasy Exome Capture V4 Probe can cover 97% and 85% of mutation sites respectively, playing an important role in disease research and diagnosis.
Stable and efficient capture rate The capture rate is over 75%.	High consistency rate of variant detection For cell line NA12878，the consistency rate of MGI Probe V4 is 99.2% and 87% for SNV and indels, respectively.
Compatible with various library preparation methods and sequencing platforms Compatible with MGIEasy Exome Universal Library Prep Kit and MGIEasy Exome FS Library Prep Kit, as well as other library preparation kits. Compatible with sequencing platforms from MGI, Illumina and Thermo Fisher.

Probe regions 59Mb, covering over 20000（refGene）genes

The databases used for probe design include CCDS、GENCODE、RefSeq、miRBase and so on.

Deep and comprehensive coverage

In HGMD and ClinVar databases, MGIEasy Exome Capture V4 Probe can cover 97% and 85% of mutation sites respectively, playing an important role in disease research and diagnosis.

Stable and efficient capture rate

The capture rate is over 75%.

High consistency rate of variant detection

For cell line NA12878，the consistency rate of MGI Probe V4 is 99.2% and 87% for SNV and indels, respectively.

Compatible with various library preparation methods and sequencing platforms

Compatible with MGIEasy Exome Universal Library Prep Kit and MGIEasy Exome FS Library Prep Kit, as well as other library preparation kits. Compatible with sequencing platforms from MGI, Illumina and Thermo Fisher.

Recommended Applications

Probes for capture	Library preparation set	Compatible sequencing	Read length	Applications
MGIEasy Exome Capture V4 Probe Set	MGIEasy Exome FS Library Prep Set (1000009658)	DNBSEQ-T7 DNBSEQ-G400	PE100/PE150	Whole exome sequencing
MGIEasy Exome Universal Library Prep Set	PE100/PE150	Whole exome sequencing
Other library preparation kits	PE100/PE150	Whole exome sequencing

Probes for capture

Library preparation set

Compatible sequencing

Read length

Applications

MGIEasy Exome Capture V4 Probe Set

MGIEasy Exome FS Library Prep Set (1000009658)

DNBSEQ-T7

DNBSEQ-G400

PE100/PE150

Whole exome sequencing

MGIEasy Exome Universal Library Prep Set

PE100/PE150

Whole exome sequencing

Other library preparation kits

PE100/PE150

Whole exome sequencing

Product Specification

Product name	MGIEasy Exome Capture V4 Probe Set
Probe lenght	90nt
Covered region size	59Mb
Databasesused for probe design	CCDSGE, NCODE, RefSeq, miRBase
Covered regions	Exome regions, >99% disease-related regions
Storage	-80℃，-25℃~-18℃，Room temperature（Refer to kit label）
DNAinput	1μg PCR product for one sample per hybridization, >250ng each sample for multiple samples per hybridizationand 1μg ≤total input≤2μg
Number of samples per hybridization	1-8
Sample types	PCR product
Compatible species	Human
Compatible sequencing platforms	Platforms from MGI，Illumina and Thermo Fisher
Read length	PE100/PE150
Detectable mutation types	SNV & Indel
Technologies	Capture+NGS
Read length	PE100/PE150