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Case 3 Zebrafish development and differentiation

The experiment was performed at six key developmental time points within 24 h of fertilization in zebrafish embryos, using Stereo-seq technology to analyze a total of 91 sagittal frozen sections, and draw a spatiotemporal transcriptome map of zebrafish early embryonic development. Stereo-seq and scRNA-seq data were integrated at each key developmental time point to construct the spatio-temporal developmental trajectory of cell fate transition and cell molecular changes during zebrafish embryonic development, and to explore the relationship between cell space microenvironment and differentiation direction associated.

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