Using DNBelab C4 and DNBSEQ sequencing platforms, single-cell transcriptome sequencing was performed on the main tissues and organs (lung, kidney, liver, pancreas, brain, aorta, thyroid, parotid gland, and blood) of cynomolgus monkeys, and established single cell transcriptome database of Macaca fascicularis. The transcriptome database has identified 44 main cell types of cynomolgus monkeys, and completed the construction of non-human primate single-cell map V1.0 for the first time in the world, which has an important guiding role in the development of new crown drugs and vaccines.