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MGIEasy Pa-SNPs Genotyping Kit

MGIEasy Pa-SNPs Genotyping Kit is developed for rapid detection of 2010 high-frequency single nucleotide polymorphisms (SNPs) of human. The kit is designed on MGI's proprietary ATOPlex platform – a multiplex PCR design platform. It includes all the required reagents to prepare sequencing libraries from DNA samples. MGIEasy Pa-SNPs Genotyping Kit can be applied to various sample types, such as cfDNA from plasma, gDNA from blood, saliva, tissue or sperm. It is specifically designed for use in forensics genomics applications, including non-invasive prenatal paternity testing, paternity testing and individual identification

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MGIEasy Pa-SNPs Genotyping Kit （96 RXN）

Item No.：1000016270

Box 1	Including PCR primer, PCR enzyme, buffer, circularization reagents, digestion reagents and anti-contamination reagents
Box 2	Including 96 PCR barcode primer
Box 3	Two types of beads for PCR product cleanup and digestion product cleanup

Features

Easy to use Two-step PCR in one tube to amplify 2010 SNPs；	Fast and efficient workflow 6.5 hours in total with only 1.5 hours hands-on time；
High sensitivity Sample input as little as 1 ng of DNA；	High accuracy Accurate detection of 2% minor contributors；
Wide range of applications Specifically designed for cfDNA and highly degraded samples.

Easy to use

Two-step PCR in one tube to amplify 2010 SNPs；

Fast and efficient workflow

6.5 hours in total with only 1.5 hours hands-on time；

High sensitivity

Sample input as little as 1 ng of DNA；

High accuracy

Accurate detection of 2% minor contributors；

Wide range of applications

Specifically designed for cfDNA and highly degraded samples.

Product Specification

Product name	MGIEasy Pa-SNPs Genotyping Kit
Version	V1.0
Reaction/kit	96 RXN/kit
Catalog No.	1000016270
Species Compatibility	Human
Sample types	cell free DNA (cfDNA) from plasma and genomic DNA (gDNA) from blood, tissues, blood spot, saliva, buccal swabs, semen, hair, and nails
Recommend Input Quantity	cfDNA, 8-20 ng cfDNA, DNA concentration ≥0.4 ng/µL; gDNA, 1-20 ng;
Library Method	Multiplex PCR
Amplicon Size	60-90 bp
Library Time	6.5 hrs
Recommended Read Length	SE50
Platform Compatibility	DNBSEQ-G400, DNBSEQ-G50
Recommended sequencing data	cfDNA, ≥25M reads; gDNA, ≥ 5M reads

Recommended MGI Process

Applications	Library preparation	Sequencing	Bioinformation analysis
Non-invasive prenatal paternity testing	MGIEasy Pa-SNPs Genotyping Kit	DNBSEQ-G400（SE50） DNBSEQ-G50(SE50)

MGIEasy Pa-SNPs Genotyping Kit