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CoolMPS High-throughput Sequencing Set

CoolMPS High-throughput Sequencing Set^（1） is a novel antibody-based sequencing product. The dNTPs of CoolMPS are without fluorescent labeled (called cold dNTPs) which incorporated into the sequencing strand by DNA polymerase, and base calling is achieved by specific binding of fluorescently labeled antibodies. During this process, the incorporated bases are unmodified, ultimately resulting in clearer base calling.

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CoolMPS High-throughput Sequencing Set (DNBSEQ-G400RS FCL SE50)

Item No.：1000017992

1000016985	DNBSEQ-G400RS Sequencing Flow Cell
1000017991	CoolMPS High-throughput Sequencing Kit (DNBSEQ-G400RS FCL SE50)

CoolMPS High-throughput Sequencing （DNBSEQ-G400RS FCL SE100）

Item No.：1000016933

1000016985

DNBSEQ-G400RS Sequencing Flow Cell

1000016934

CoolMPS High-throughput Sequencing Kit (DNBSEQ-G400RS FCL SE100)

CoolMPS High-throughput Sequencing （DNBSEQ-G400RS FCL PE100）

Item No.：1000016935

1000016985	DNBSEQ-G400RS Sequencing Flow Cell
1000016936	CoolMPS High-throughput Sequencing Kit (DNBSEQ-G400RS FCL PE100)

CoolMPS High-throughput Sequencing （DNBSEQ-G400RS Small RNA FCL SE50）

Item No.：1000019478

1000016985

DNBSEQ-G400RS Sequencing Flow Cell

1000017991

CoolMPS High-throughput Sequencing Kit (DNBSEQ-G400RS FCL SE50)

1000006387

MGIEasy Wash Buffer For Small RNA Sequencing

Features

Labeled antibodies lead to higher signal Multiple labels per antibody, stronger signals	No quenching in CoolMPS Natural base, no “scar” induced quenching
Polymerase provided natural base environment for binding and incorporation, reaction closer to completeness Closer to 100% reaction>>Less signal decay>>Great potential for longer reads

Labeled antibodies lead to higher signal

Multiple labels per antibody, stronger signals

No quenching in CoolMPS

Natural base, no “scar” induced quenching

Polymerase provided natural base environment for binding and incorporation, reaction closer to completeness

Closer to 100% reaction>>Less signal decay>>Great potential for longer reads

Recommended Applications

Type	Part number	Kit name
Pair end sequencing (PE100) for MGI dual index library	1000011532	CPAS Barcode Primer 3
Pair end sequencing (PE100) for MGI dual index library	1000016935	CoolMPS High-throughput Sequencing Set (DNBSEQ-G400RS FCL PE100)
Single end sequencing (SE50) for App-A single index library	1000017992	CoolMPS High-throughput Sequencing Set (DNBSEQ-G400RS FCL SE50)
Single end sequencing (SE50) for App-A single index library	1000012291	High-Throughput Single-End Sequencing Primer Kit (App-A)
Single end sequencing (SE100) for App-A single index library	1000016933	CoolMPS High-throughput Sequencing Set (DNBSEQ-G400RS FCL SE100)
	1000012291	High-Throughput Single-End Sequencing Primer Kit (App-A)
Pair end sequencing (PE100) for App-A single index library	1000016935	CoolMPS High-throughput Sequencing Set (DNBSEQ-G400RS FCL PE100)
Pair end sequencing (PE100) for App-A single index library	1000004156	High-Throughput Pair-End Sequencing Primer Kit (App-A)
Pair end sequencing (PE100) for App-A dual index library	1000016935	CoolMPS High-throughput Sequencing Set (DNBSEQ-G400RS FCL PE100)
	1000004156	High-Throughput Pair-End Sequencing Primer Kit (App-A)
	1000014047	High-Throughput Barcode Primer 3 Reagent Kit(App-A)

Product Specification

Flow cell	FCL
Read length	SE50	SE100	PE100
Maximum cycles	70	120	220
Effective reads/flow cell	1500～2000M	1500～2000M	1500～2000M
Data output/flow cell	75～100G	150～200G	300～400G
Q30*	>90%	>90%	>90%
Run time**	～17H	～30H	～58H
* Effective Reads and Q30 are based on the standard library. These values may vary with various applications. ** Run time includes DNB loading, sequencing, and data process (Fastq writing). Run time may vary with various instruments.