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MGIEasy Exome Capture V5 Probe Set

MGIEasy Exome Capture V5 Probe Set not only covers the regions of traditional exome probes, but also ensures the comprehensive capture of coding sequences related to various diseases by targeted design, e.g. reproductive, neonatal, cardiovascular and cerebrovascular, hereditary tumors/deafness, monogenic, medication safety, personal genome, immunodeficiency, mitochondrial defects etc. The primary targets with a design size of 69Mb include: CCDS, GENCODE, RefSeq, miRbase and MGI Clinical Database. As a universal exome capture probe set, it is compatible with both of MGI and other NGS platforms.

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MGIEasy Exome Capture V5 Probe Set（16 RXN）

Item No.：940-000187-00

Probes

MGIEasy Exome Capture V5 Probe（16RXN）

1000007741

Hybridization and elution

MGIEasy Exome Capture Hybridization and Wash Kit (Box 1) （16RXN）

940-000168-00

Hybridization and elution

MGIEasy Exome Capture Hybridization and Wash Kit (Box 2) （16RXN）

940-000169-00

Features

Probe regions 69Mb

The databases used for probe design include CCDS、GENCODE、RefSeq、miRBase and so on. It also ensures the comprehensive capture of coding sequences related to various diseases by targeted design, e.g. reproductive, neonatal, cardiovascular and cerebrovascular, hereditary tumors/deafness, monogenic, medication safety, personal genome, immunodeficiency, mitochondrial defects etc.

More comprehensive gene coverage

455 genes can be 100% covered.

Superior coverage and uniformity

When the mean sequencing depth is 100X, 96% of the regions can reach coverage of 20X.

Stable and efficient capture rate

The capture rate is over >60% (by reads).

Probe regions 69Mb

More comprehensive gene coverage

455 genes can be 100% covered.

Superior coverage and uniformity

When the mean sequencing depth is 100X, 96% of the regions can reach coverage of 20X.

Stable and efficient capture rate

The capture rate is over >60% (by reads).

Recommended Applications

Probes for capture	Library preparation set	Compatible sequencing	Read length	Applications
MGIEasy Exome Capture V5 Probe Set	MGIEasy Exome FS Library Prep Set (1000009658)	DNBSEQ-T7 DNBSEQ-G400	PE100/PE150	Whole exome sequencing
MGIEasy Exome Universal Library Prep Set	PE100/PE150	Whole exome sequencing
Other library preparation kits	PE100/PE150	Whole exome sequencing

Probes for capture

Library preparation set

Compatible sequencing

Read length

Applications

MGIEasy Exome Capture V5 Probe Set

MGIEasy Exome FS Library Prep Set (1000009658)

DNBSEQ-T7

DNBSEQ-G400

PE100/PE150

Whole exome sequencing

MGIEasy Exome Universal Library Prep Set

PE100/PE150

Whole exome sequencing

Other library preparation kits

PE100/PE150

Whole exome sequencing

Product Specification

Kit name	MGIEasy Exome Capture V5 Probe Set
Probe lenght	90nt
Covered region size	69Mb
Databases used for probe design	CCDS, GENCODE, RefSeq, miRBase, MGI Clinical Database
Covered regions	Traditional exome regions and various diseases related regions
Storage	-80℃，-25℃~-18℃，Room temperature（Refer to kit label）
DNA input	1μg PCR product for one sample per hybridization, >250ng each sample for multiple samples per hybridization and 1μg ≤total input≤2μg
Number of samples per hybridization	1-8
Sample types	PCR product
Compatible species	Human
Compatible sequencing platforms	Platforms from MGI，Illumina and Thermo Fisher
Read length	PE100/PE150
Detectable mutation types	SNV & Indel
Technologies	Capture+NGS