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ATOPlex RNA Library Prep Set

ATOPlex RNA Library Prep Set includes RT and two-steps PCR that convert viral RNA to sequence libraries in 5 hours. With unique technology, ATOPlex performs high specificity of amplification and low dimer rate. Moreover, the ATOPlex kit includes clean system and spike-in system which minimizes cross-contamination risk and improves the accuracy of virus loads quantification. It is suitable for population-scale virus detection, surveillance, and tracing.

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ATOPlex RNA Library Prep Set

Item No.：940-000183-00

RT and PCR Reagment	ATOPlex RNA Universal Library Preparation Module	940-000181-00
Primer of SARS-CoV-2	Internal Control Primer Pool	940-000241-00
Dual Barcode Primer	ATOPlex Dual Barcode Primer Module (01-96)	1000021626
Dual Barcode Balance Library	ATOPlex Dual Barcoded Balance Library Reagent	1000022270

Features

Ultra-sensitive Detection Ultra-sensitive Detection with as Low as 10 Copies/mL Viral Loads	Full-length Genome Coverage >99% Full-length Genome Coverage with Challenge Sample
Simple operation Easy Protocol with RT and Two-step PCR in One Tube	Accurate quantification Accurate Quantification by Spike-in Control

Ultra-sensitive Detection

Ultra-sensitive Detection with as Low as 10 Copies/mL Viral Loads

Full-length Genome Coverage

>99% Full-length Genome Coverage with Challenge Sample

Simple operation

Easy Protocol with RT and Two-step PCR in One Tube

Accurate quantification

Accurate Quantification by Spike-in Control

Product Specification

Product Name	ATOPlex RNA Library Prep Set
Configuration	96 Preps/kit
Sample Types	Total Nucleic Acid from Nasopharyngeal Swabs, BALF, etc
Application	Surveillance, Variation and Evolution Analysis of SARS-CoV-2
Detection Region	SARS-CoV-2 Full Length
Amplicons Size	106-199 bps
Amplicons Numbles	273 Amplicons in one tube
cDNA input	>10 copies genome for full length，>10 copies/mL for detection
Variant Types	SNP，InDel
Total Time（sample to library）	5 hrs
Uniformity（0.1X）	95%
On Target Aligned Reads	≥95%
Coverage	≥99%
Recommend Sequence Type	PE100 for Full Length Genome，SE50 for Detection
Recommend Total Reads	0.5-3 M Reads/sample

Recommended MGI Process

Applications	Library preparation	Sequencing	Bioinformation analysis
Surveillance, Variation and Evolution Analysis of SARS-CoV-2	ATOPlex RNA Library Prep Set	DNBSEQ-T7(PE100) DNBSEQ-G400(PE100)	Fast identification of pathogenic infections(PFI) and MGAP（Microbial Genome Analysis Pipeline） MGI-SARS-CoV-2 pipeline ATOPlex SARS-CoV-2 software

Applications

Library preparation

Sequencing

Bioinformation analysis

Surveillance, Variation and Evolution Analysis of SARS-CoV-2

ATOPlex RNA Library Prep Set

Fast identification of pathogenic infections(PFI) and MGAP（Microbial Genome Analysis Pipeline）
MGI-SARS-CoV-2 pipeline
ATOPlex SARS-CoV-2 software

ATOPlex RNA Library Prep Set