Location: Home /  About MGI

Powered also by powerful kits! --- MGIEasy RNA Library Prep Set

Release date:2018-10-17Writer:MGIViews:2907Share

Besides genetic sequencers, various library preparation products have become more and more available and play an essential role in advancing the development of sequencing. MGI has been developing a variety of library preparation kits too, along with its own sequencing platforms.


As one of MGI RNA libraries preparation series, MGIEasy RNA Library Prep Set provides an efficient package for generating libraries from a wide range (10 ng -1 ug) of total RNA that is suitable for MGI high-throughput sequencing platforms.


It is easy to use and can be widely applied to human, animal, plant and microbe transcriptome research for comprehensive and rapid analysis of gene expression changes, examining rare and novel transcripts, the discovery of alternative splicing events, gene fusions, SNPs and allele-specific expression.


Data performance

Libraries were prepared from an input of Universal Human Reference RNA(UHRR) ranging from 10 ng -1 μg using the MGIEasy RNA Library Prep Set and sequenced on MGISEQ-2000 at PE100 read-length.


Across all input amounts of total RNA (1 ug, 200 ng, 50 ng, 10 ng), MGIEasy RNA Library Prep Set exhibits excellent quality sequencing data and transcript annotation at genome mapping, gene mapping rate, the numbers of genes or transcripts detected, and concordance of gene expression.


Fig. 1 Comparison of the sequencing data and transcript annotation under different input amount of total RNA.


Fig. 2 Gene expression level reproducibility and concordance in different input amounts of total RNA


Uniformity of coverage across the entire transcript is important for analysis of gene structure. A comparison was performed between the MGIEasy RNA Library Prep Set sequenced on an MGISEQ-2000 and other comparable RNA kits sequenced on another platform. The results demonstrate that the libraries constructed using MGIEasy RNA Library Prep Set have superior 3’end coverage.


Fig.3 Reads randomness of different platforms and kits

  • Hotline (Global)
    +86-4000-688-114
  • Email
    MGI-service@mgi-tech.com
  • Special Recommendations
  • *For research use only.

    Not for use in diagnostic procedures (except as specifically noted).
Copyright © 2024 All Rights Reserved MGI Tech Co., Ltd. Guangdong ICP 16117185Privacy PolicyLegal NoticesCookie Policy
Our use of cookies
We would like to use necessary cookies to improve your browsing experience and the quality of our website. We would also like to set analytics cookies and advertisement cookies that help us make improvements by measuring how you use our website. Detailed information about the use of cookies on this website and how you can control your consent can be found in our Cookie Policy and Privacy Policy.
Accept only strictly necessary cookiesAccept all cookies