Product FAQs | DNBSEQ-E25

The "Product FAQs" column collects products related questions which MGI customers inquiries frequently. And we organizes FAQs which will be released in the future to help you understand and use MGI products better.

Q1:What are the unique highlights of DNBSEQ-E25?

DNBSEQ-E25, compact, simple, fast, use 0 threshold:

◾Compact: 0.1 square meters; no clean room required; no special environmental requirements.

◾Simple: ready to use out of the box; no need for optical path adjustment; easy to maintain.

◾Fast: 10-minute prep time; 5-8 hours of sequencing.

Q2:DNBSEQ-E25 footprint?

The DNBSEQ-E25 instrument occupies a table area of only 0.1 square meters.

Q3:Which applications are the DNBSEQ-E25 suitable for?

The main applications include rapid unknown pathogen detection, small whole genome sequencing, targeted next-generation sequencing, education and forensic, etc.

Q4:Maximum capacity of DNBSEQ-E25?

The DNBSEQ-E25 has a maximum capacity of 7.5GB.

Q5:What is the fastest time for DNBSEQ-E25 to output downlink data?

DNBSEQ-E25 can output downstream data (SE100 sequencing read length) in about 5 hours at the earliest, which can give immediate response to the urgent sequencing needs of special samples, and is suitable for application scenarios such as the handling of public health emergencies.

Q6:Minimum and maximum read length for DNBSEQ-E25?

The minimum reading length of DNBSEQ-E25 is SE35, and the maximum reading length is PE150. you can also customize the reading length in the instrument operation interface, such as SE75, PE100, etc.

Q7:Does the DNBSEQ-E25 support customized read lengths in addition to the maximum read length?

With this support, the operator can enter the desired read lengths into the instrument interface, for example, SE35, SE50, SE75 read lengths using SE100 consumables, PE100 sequencing using PE150 consumables, or double-ended unequal sequencing applications, e.g. Read1: 60bp+Read2: 30bp.

Q8:How many models of DNBSEQ-E25 are available?

DNBSEQ-E25 is available in 2 configurations, including: the standard model (DNBSEQ-E25RS) which can efficiently process the raw data collected by the host computer and output FASTQ data in standard format; and the extended model (DNBSEQ-E25ARS) which can integrate the raw information process on the basis of the standard model, realizing the sequencing and analysis in one. 

Q9:Is the DNBSEQ-E25 sequencer compatible with UWI's lab management system?

Similar to other sequencers of MGI, DNBSEQ-E25 sequencer can realize automatic transmission of sequencing data through Zlims system. It can also be used for scheduling and analyzing raw information through the Zlims system.

Q10:How is DNBSEQ-E25 similar to other DNBSEQ platforms in terms of biochemical principles?

The similarities and differences between DNBSEQ-E25 and other DNBSEQ platforms in terms of biochemical principles are as follows:

(1) Same: DNBSEQ-E25 adopts MGI unique DNBSEQ™ sequencing technology, including rolled-loop amplification to generate DNA nanoballs (DNBs), regular-array carriers, and cPAS combined probe-anchored polymerization sequencing method, which features high sequencing accuracy, low rate of repetitive sequences, and fewer label jumps.

(2) Differences:

① Fluid: DNBSEQ-E25 adopts microfluidic technology, integrating the liquid flow path in traditional sequencers into a disposable chip, which saves reagent consumption, and the waste liquid is discarded along with the consumables after the test, which is simple to operate, and there is no liquid contact inside the instrument, which eliminates the need for daily cleaning and maintenance, and significantly improves the reliability of sequencers.

② Detection: DNBSEQ-E25 adopts CMOS sensor to read DNA sequence information directly from DNB array at the same time, no need to rely on the traditional optical system, significantly shorten the sequencing time.

③Chemistry: DNBSEQ-E25 adopts an improved version of cPAS co-probe-anchored polymerization sequencing method to accelerate the biochemical reaction rate and further shorten the sequencing time.

Q11:Does the DNBSEQ-E25 kit support single and dual Barcode sequencing?

Yes, all models of sequencing consumables are pre-loaded with barcode primers and sequencing reagents for Barcode sequencing (Single or Dual Barcode sequencing, up to 20bp), and there is no need to add any additional reagents. Therefore，the SE100 and PE150 consumables are available in 120Cycles and 320Cycles sizes.

Q12:None of the times within the DNBSEQ-E25 parameters include label sequencing time, how much additional time is needed if Barcode is measured?

Depending on the barcode read length (8bp or 10bp), single Barcode sequencing will take an additional 35-40 minutes and double Barcode sequencing will take an additional 60-70 minutes.

Q13:How is the DNBSEQ-E25 Sequencing Kit similar or different from other platforms in terms of the DNB preparation process?

By default, DNBSEQ-E25 is adapted to one-step preparation of DNB, which is directly prepared from linear libraries (dsDNA, such as PCR products, ligation products, etc.). Compared with the two-step method of single-stranded cyclization and then preparation of DNB, the time for library construction and preparation of DNB can be further compressed. This design mainly takes into account the fact that the E-sequence is mainly used for rapid sequencing, which needs to compress the time of library construction and sequencing process as much as possible, and also takes into account the adaptability of the DNBelab-D4 digital sample preparation system and the DNBSEQ-E25 sequencer.

Q14:How to maintain DNBSEQ-E25?

Due to the design of the microfluidic sequencing carriers and integrated reagent cartridges, the DNBSEQ-E25 sequencer does not require any additional water washing or other operations under normal operation. We recommend that you wipe the E25 carrier tray with a cotton ball moistened with 75% alcohol at least once a month while the E25 is powered on and make sure that there is no liquid or crystalline residue left on the carrier tray.

Q15:What if I need to pack DNBSEQ-E25 for transportation to another lab site?

Before packing, please turn off the calculation module by selecting Calculation Module Shutdown in the instrument interface, and then place the transportation carrier sheet on the carrier table and select "Packing Shutdown" in the instrument interface, the instrument will run automatically and reset to the packing position. When the interface of sequencer main unit shows the interface of power off, turn off the power of sequencer main unit. After that, pack the sequencer main unit, computing module, power adapter, power cord, etc. in order.