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MGIEasy Fast PCR-FREE FS Library Prep Set V2.0

The MGIEasy Fast PCR-FREE FS Library Prep Set V2.0 is designed to prepare WGS PCR-Free library for DNBSEQ^TM sequencers. This library prep set is optimized to convert 25 ng~900 ng genomic DNA (gDNA) into a special library and uses high quality fast fragmentase to combine the fragmentation, end-repair and add A into one step, to simplify the preparation process and significantly shorten the duration of DNA library preparation. Fast PCR-FREE combined with MGI DNBSEQ^TM sequencer, is truly PCR-free sequencing with no amplification errors accumulation.

The PCR-free method and optimized library prep make it perform well in genome coverage uniformity and mutation detection precision and sensitivity. The set combined with the MGISP-100 and MGISP-960, could provide automatic library preparation workflows. This library prep set is suitable for WGS with fast, easy-to-use, and excellent performance and is the preferred recommendation for the large genome sequencing project.

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MGIEasy Fast PCR-FREE FS Library Prep Set V2.0 (16 RXN)

Item No.：940-000886-00

Component Kits	Component Kits Name & Item No.	Quantity
Core library	MGIEasy Fast PCR-FREE FS Library Prep Module V2.0 (16 RXN) Item No.: 940-000885-00	1
Adapter	MGIEasy UDB PF Adapter Kit (16 barcode,16 RXN) Item No.: 940-000018-00	1
Clean Beads	MGIEasy DNA Clean Beads (3.2 mL beads) Item No.: 940-001176-00	1

MGIEasy Fast PCR-FREE FS Library Prep Set V2.0 (96 RXN)

Item No.：940-000884-00

Component Kits	Product Name & Item No.	Quantity
Core library	MGIEasy Fast PCR-FREE FS Library Prep Module V2.0 (96 RXN) Item No.：940-000883-00	1
Adapter	MGIEasy UDB PF Adapter Kit A (96 barcode,96 RXN) Item No.：940-000023-00	1
Clean Beads	MGIEasy DNA Clean Beads (15 mL beads) Item No.：940-001174-00	1

MGIEasy Fast PCR-FREE FS Library Prep Set V2.0 (384 RXN)

Item No.：940-000882-00

Component Kits	Component Kits Name & Item No.	Quantity
Core library	MGIEasy Fast F PCR-FREE S Library Prep Module V2.0 (96 RXN) Item No.: 940-000883-00	4
Adapter	MGIEasy UDB PF Adapter Kit A (96 barcode,96 RXN) Item No.: 940-000023-00	1
	MGIEasy UDB PF Adapter Kit B (96 barcode,96 RXN) Item No.: 940-000022-00	1
	MGIEasy UDB PF Adapter Kit C (96 barcode,96 RXN) Item No.: 940-000025-00	1
	MGIEasy UDB PF Adapter Kit D (96 barcode,96 RXN) Item No.: 940-000024-00	1
Clean Beads	MGIEasy DNA Clean Beads (50 mL beads) Item No.: 1000005279	1

Features

Fast library prep workflow Combine the DNA fragmentation, end repair and add A into one step, significantly reducing the library prep time in 1.3 hours.	Flexible workflow to accommodate variations in sample type, DNA input amount, application Compatible with 25 ng~900 ng DNA input, supporting variety sample types, including blood, saliva, oral swabs and virus long amplicons，suitable for high and low depth WGS of various species, as well as microbial sequencing and pathogen amplicon detection.
High uniform and consistent insert sizes across a wide DNA input range and sample types Using a high-quality, low bias enzyme for different species and input amounts shearing with the same incubation condition can get the high uniform and consistent insert sizes.	Excellent performance High uniform genome coverage, better coverage of GC-rich regions. Outstanding mutation detection precision and sensitivity performance.
Simple and automated workflow Minimal operation steps, simple and easy to use. Compatible with automatic sample preparation systems to provide automated workflow.

Fast library prep workflow

Combine the DNA fragmentation, end repair and add A into one step, significantly reducing the library prep time in 1.3 hours.

Flexible workflow to accommodate variations in sample type, DNA input amount, application

Compatible with 25 ng~900 ng DNA input, supporting variety sample types, including blood, saliva, oral swabs and virus long amplicons，suitable for high and low depth WGS of various species, as well as microbial sequencing and pathogen amplicon detection.

High uniform and consistent insert sizes across a wide DNA input range and sample types

Using a high-quality, low bias enzyme for different species and input amounts shearing with the same incubation condition can get the high uniform and consistent insert sizes.

Excellent performance

High uniform genome coverage, better coverage of GC-rich regions. Outstanding mutation detection precision and sensitivity performance.

Simple and automated workflow

Minimal operation steps, simple and easy to use. Compatible with automatic sample preparation systems to provide automated workflow.

Product Specification

Version	V2.0
Assay Time	~ 1.3 hrs -1.4 hrs
Hands-On Time	~20 min
Input Quantity	25 ng ~900 ng gDNA
Barcode	~384 Unique Dual Barcodes
Insert size	Two sides beads purification: 400 bp~600 bp One side beads purification: 600 bp~850 bp
Sample types	gDNA from blood, saliva, oral swabs, tissue, Microbial gDNA, Meta DNA, Virus long amplicons
Species Compatibility	Human, Animal, Plant, Microbe
Applications	Human WGS, Plants & animals WGS, Low-pass WGS, Microbial WGS, Metagenomics sequencing, Virus long amplicons sequencing
Platform Compatibility	DNBSEQ-G400, DNBSEQ-G50, DNBSEQ-T7, DNBSEQ-T10×4, DNBSEQ-T20×2, DNBSEQ-G99, DNBSEQ-E25
Recommended Read Length	SE100/PE100/PE150
Adaptation automation platform	MGISP-100(16RXN)，MGISP-960(96RXN, 384RXN)

Recommended MGI Process

Applications	Library preparation	Sequencing	Data analysis
Human WGS sequencing (High depth)	MGISP-100/MGISP-960 MGIEasy Fast PCR-FREE FS Library Prep Set V2.0 MGIEasy Dual Barcode Circularization Kit	DNBSEQ-G400 DNBSEQ-T7 DNBSEQ-T10×4 DNBSEQ-T20×2	MegaBOLT Bioinformatics analysis accelerator - WGS Germline variant calling pipeline - WGS Somatic variant calling pipeline
Low-pass WGS	MGISP-960 MGIEasy Fast PCR-FREE FS Library Prep Set V2.0 MGIEasy Dual Barcode Circularization Kit	DNBSEQ-G400 DNBSEQ-T7

Related Products

MGIEasy Dual Barcode Circularization Kit

Application: Prepare the double-stranded DNA library with MGl dual barcode adapter into a single-stranded circular DNA library for DNBSEQ seouencers. Applicable to the double-stranded DNA ibraries with MG1 dual barcode adapter from all MGl dual barcode librarv prep kits.

Platform Compatibility: All DNBSEQ

Reaction/kit: 16 RXN