Go Back / Location：Home / Products

MGIEasy Whole Genome Methylation Sequencing Library Prep Kit V3.0

DNA methylation is an important epigenetic mechanism that cells used to control the temporal and spatial expression of genes. MGIEasy Whole Genome Methylation Sequencing Library Prep Kit V3.0 is designed to prepare fragmented, size-selected DNA into libraries specifically for MGI high-throughput sequencing platforms. Paired with the upgraded algorithm of the MGI high-throughput sequencing platforms, it can not only greatly reduce the mixing ratio of balanced library or achieve no need to add any balanced library when sequencing, but also help to improve data yield. It provides an efficient and accurate method for genome-wide methylation library construction and sequencing. It can analyze the DNA methylation status at single base level, and thus enable the construction of a fine genome-wide methylation map.

Request Quote

MGIEasy Whole Genome Methylation Sequencing Library Prep Kit V3.0（16 RXN）

Item No.：940-001530-00

Component kits	Product Name of component kit	Part No. of component kit	Quantity of component kit in a Set
Core library & Adapter	MGIEasy Whole Genome Methylation Sequencing Library Prep Module (16 RXN)	940-001529-00	1
Circularization	MGIEasy Circularization Module (16 RXN)	1000005260	1
Clean Beads	MGIEasy DNA Clean Beads (15mL beads)	940-001174-00	1

MGIEasy Whole Genome Methylation Sequencing Library Prep Kit V3.0（96 RXN）

Item No.：940-001527-00

Component kits	Product Name of component kit	Part No. of component kit	Quantity of component kit in a Set
Core library	MGIEasy Whole Genome Methylation Sequencing Library Prep Module (96 RXN)	940-001528-00	1
Adapter	MGIEasy Methylation Adapter and Single Barcode Primer Module (96 RXN)	940-001531-00	1
Circularization	MGIEasy Circularization Module (96 RXN)	1000017573	1
Clean Beads	MGIEasy DNA Clean Beads (75mL beads)	940-001526-00	1

Features

Compatible with various sample types Compatible with sample types including human gDNA extracted from blood, fresh tissue and cells samples and FFPE samples, and cell free DNA from plasma samples, and gDNA from common plant and animal.	No need to add balanced library Paired with the upgraded algorithm of the MGI high-throughput sequencing platforms, it can achieve no need to add any balanced library when sequencing and help to improve data yield.
Superior data performances High data utilization rate, high genome coverage and accurate methylation detection.

Compatible with various sample types

Compatible with sample types including human gDNA extracted from blood, fresh tissue and cells samples and FFPE samples, and cell free DNA from plasma samples, and gDNA from common plant and animal.

No need to add balanced library

Paired with the upgraded algorithm of the MGI high-throughput sequencing platforms, it can achieve no need to add any balanced library when sequencing and help to improve data yield.

Superior data performances

High data utilization rate, high genome coverage and accurate methylation detection.

Product Specification

Application	Genome-wide methylation sequencing
Conversion method	Compatible with enzymatic conversion method to convert unmethylated C to U
DNA Fragmentation method	Covaris (no fragmentation for cfDNA)
Library construction	Double stranded DNA library construction method
Sample types	Human gDNA extracted from blood, fresh tissue and cells samples and FFPE samples, and cell free DNA from plasma samples gDNA from common plant and animal (such as mouse, Arabidopsis, etc.)
Input Quantity	100 ng~1000 ng gDNA 5 ng~20 ng cfDNA
insert size	single side size selection：350 bp ~ 480 bp dual side size selection：350 bp ~ 450 bp
Barcode	~96 single barcodes
Circularization method	single strand DNA circularization method
Assay Time	~10.5 h (from gDNA to ssCir DNA)
Sequencing platform	DNBSEQ-G400 (ECR 7.0 and later)
Recommended Read Length	PE100/PE150 (V3.1)

Recommended MGI Process

Applications	Library preparation	Sequencing	Data analysis
Human Whole Genome Methylation Sequencing	MGIEasy Whole Genome Methylation Sequencing Library Prep Kit V3.0	MGISEQ-G400	MegaBOLT-MethyScope Software

Related Products

MGIEasy Fast RNA Library Prep Set

Application: Whole-Transcriptome Sequencing, mRNA Sequencing, RNA-Seq, Metatranscriptome Sequencing

Platform Compatibility: DNBSE-G400, DNBSEQ-G50, DNBSEQ-G99, DNBSEQ-T7, DNBSEQ-T10×4

Reaction/kit: 16 RNX,96 RNX,192 RXN

MGIEasy rRNA & Globin Depletion Kit V1.3

Application: Efficiently deplete rRNA and Globin mRNA from human blood total RNA and keep other mRNA and non-coding RNA.

Reaction/kit: 16 RXN, 96 RXN

MGIEasy rRNA Depletion V1.3

Application: Efficiently deplete rRNA from human, mouse and rat total RNA and keep mRNA and non-coding RNA.