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MGIEasy PCR-Free DNA Library Prep Set

MGIEasy PCR-Free Library Prep Set is specifically designed for making WGS libraries for MGI High-throughput Sequencing Platforms. This library prep set is optimized to convert 80-200 ng size selected fragmented DNA into a customized library. MGI WGS PCR-free library preparation, combined with MGI DNBSEQ^TM sequencer, is the true PCR-free NGS workflow without PCR amplification, resulting in no error accumulation and higher data accuracy.

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MGIEasy PCR-Free DNA Library Prep Set (16 RXN)

Item No.：1000013452

Library and Circularization

MGIEasy PCR-Free DNA Library Prep Kit

1000013456

Adapter

MGIEasy PF Adapters-16（tube）Kit

1000013460

Beads

MGIEasy DNA Clean Beads (8 mL)

940-001596-00

MGIEasy PCR-Free DNA Library Prep Set (96 RXN)

Item No.：1000013453

Core library	MGIEasy PCR-Free DNA Library Prep Kit	1000013457
Adapter	MGIEasy PF Adapters-96 (plate) Kit	1000013461
Beads	MGIEasy DNA Clean Beads (50 mL)	940-001594-00

Features

Compatible with Multiple Species Types Compatible with human, animals, plants, bacteria, fungi etc., e.g. human (blood, saliva, fresh tissue), mice, rice, E.coli and metagenomics.	Fast, Simple and Automated Workflow Library preparation can be completed in 3.5 hours with size-selected DNA as input; the workflow is simple and suitable for automated library preparation without the risk of amplicon contamination.
No Amplification Error Accumulation By eliminating PCR amplification steps, WGS PCR-free prepared and sequenced by MGI DNBSEQ^TM platform has no amplification error accumulation, resulting in better genome fidelity.	Higher Coverage Uniformity Compared with traditional WGS (PCR amplification), WGS PCR-free reduces GC bias and improves coverage uniformity across the genome, such as GC-rich region, promoter and repetitive region.
Excellent Performance of Variant Detection Compared with traditional WGS (PCR amplification), PCR-free WGS shows higher sensitivity and accuracy of variant detection, especially indels.

Compatible with Multiple Species Types

Compatible with human, animals, plants, bacteria, fungi etc., e.g. human (blood, saliva, fresh tissue), mice, rice, E.coli and metagenomics.

Fast, Simple and Automated Workflow

Library preparation can be completed in 3.5 hours with size-selected DNA as input; the workflow is simple and suitable for automated library preparation without the risk of amplicon contamination.

No Amplification Error Accumulation

By eliminating PCR amplification steps, WGS PCR-free prepared and sequenced by MGI DNBSEQ^TM platform has no amplification error accumulation, resulting in better genome fidelity.

Higher Coverage Uniformity

Compared with traditional WGS (PCR amplification), WGS PCR-free reduces GC bias and improves coverage uniformity across the genome, such as GC-rich region, promoter and repetitive region.

Excellent Performance of Variant Detection

Compared with traditional WGS (PCR amplification), PCR-free WGS shows higher sensitivity and accuracy of variant detection, especially indels.

Product Specification

Product Name	MGIEasy PCR-Free DNA Library Prep Set
Application	Whole genome sequencing
The version of Set	V1.1
Configuration	16 RXN（1000013452） and 96 RXN（1000013453）
Validity period	9 month
Assay Time	~3.5 hrs
Input Quantity	80-200 ng fragment DNA
Sample types	gDNA
Species Compatibility	Human, animals,plants, fungi, bacteria, metagenomics, etc.
Fragmentation Method	Sonication
Recommended Insert Size	350-400 bp
Platform Compatibility	DNBSEQ-G400, DNBSEQ-G50, DNBSEQ-T7
Recommended Read Length	SE100, PE100, PE150, PE200, SE400

Recommended MGI Process

Application	Library Preparation	Sequening	Analysis
Human WGS sequencing (High depth)	MGIEasy PCR-Free DNA Library Prep Set MGISP-100 MGISP-960	DNBSEQ-G400* DNBSEQ-G50* DNBSEQ-T7*	MegaBOLT Bioinformatics analysis accelerator -WGS germline mutation analysis software -WGS somatic mutation analysis software

Application