In face of the war against novel coronavirus, MGI’s DNBSEQ series of high-throughput sequencers are used in pneumonia prevention phenomena and control such as genome sequence assembly, suspected patient detection, and mutation detection.

DNBSEQ-G400 became one of the first coronavirus genome sequencers to be released.

DNBSEQ-G400, as a high-throughput sequencer, is widely used in clinical and scientific research. It plays an important role in sequencing localized data across the country and helping to analyze the genome sequence of the new coronavirus virus.

DNBSEQ-G400

On December 30, in the earliest batch of 13 new types of coronavirus genome sequence data submitted to the global shared influenza virus database GISAID, five cases of data were obtained from MGI's high-throughput sequencer DNBSEQ-G400. The study and analysis of the evolutionary sources and pathogenesis of the new coronavirus in Wuhan provided first-hand information.

On January 23, DNBSEQ-G400 played a vital role in the emergency detection efforts in Beijing. After receiving the mission, it quickly built a database and got on the machine, and successfully finished the job at 3 am on January 25. At the same time, In the global shared influenza virus database GISAID, the genome sequence of the new coronavirus has been published up to 26 cases

New coronavirus 2019-nCoV genome structure diagram

(Source: Nextstrain)

On January 25th, in a Shenzhen hospital, DNBSEQ-G400 was used for traceability analysis and research to meet the needs of clinical diagnosis and treatment.

On January 27th, DNBSEQ-G400 located in Beijing was used once again and proved its comprehensive flexibility, stable quality, and fast sequencing speed. As a supplement to the nucleic acid detection kit (PCR method), for some samples with low virus content, based on DNBSEQ-G400 high-throughput sequencer for sequencing can improve the positive detection rate and avoid missed detection and false detection; for suspected infection, multiple infections or secondary infection pathogen samples, and DNBSEQ high-throughput sequencer for sequencing, you can perform concurrent testing provides more information about the pathogens that may be infected.

Ultra-high-throughput sequencer DNBSEQ-T7 becomes a large-scale detection equipment

At the end of the year, Wuhan, with the cooperation of the Institute of Microbiology, Chinese Academy of Sciences, Shandong First Medical University, quickly completed the sequencing of the new coronavirus group.

On January 21, the newly-added DNBSEQ-T7 sequencer was deployed overnight, which can achieve the output from 128 samples to the next report in 128 cases in the fastest time, and the total of each sample obtained by high-throughput sequencing. The number of reads exceeds 100 million reads, which can effectively improve the detection sensitivity of pathogenic microorganisms.

Domain application experts perform operations before DNBSEQ-T7

In addition, on January 22, DNBSEQ-G50, a DNBSEQ high-throughput sequencer located in Shanghai, Guangzhou and other places, also completed data update, and the updated database contains new coronavirus sequences.

MGI established a special emergency response team and opened a technical support line

In order to better support the operation of sequencers in various regions, MGI established an emergency response team and opened an emergency technical support line to stand by 24 hours to jointly help the epidemic prevention and control. Equipped with elite forces throughout the country and stand by 24 hours to provide high-throughput. The new coronavirus emergency plan for sequencing methods supports clinical identification and provides diagnostic basis for other coronaviruses and respiratory pathogen infections, including new coronaviruses, to achieve rapid detection of viral sequences, and to provide support for monitoring possible virus mutations.

MGI also opened an emergency technical support line (4000-966-988, press 5 to enter the voice prompt to call), and fully assist the epidemic prevention and control.

Contact of MGI 2019-nCoV High-throughput Sequencing Package    

Dr. Jiang, 15972014336

Leaders of the 2019-nCoV emergency team in China

North China: Mr. Du, 18911279162

East China: Mr. Zhao, 17621034555

South China: Mr. Zeng, 18718924024

Central China: Mr. Jiang, 13681802511

[1] DNBSEQ-G400 and DNBSEQ-G50 remain their original product names as MGISEQ-2000 and MGISEQ-200 respectively in China and some other overseas countries.