MegaBOLT: MGI Bioinformatics analysis accelerator Software - Advanced Tools -MGI Tech website-Leading Life Science Innovation

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Product Description

MegaBOLT Bioinformatics Analysis Accelerator Series (hereinafter called MegaBOLT) is an MGI-developed MPS-dedicated acceleration system for bioinformatics analysis.

MegaBOLT supports analysis of Whole Genome Sequencing (WGS), Whole Exome Sequencing (WES) and Panel Sequencing on Germline or Somatic data, which can be 100 times faster than classical algorithms.

ZMART, the bioinformatics application market integrated with MegaBOLT, supports more than 100 add-on applications to provide extended functionality. MegaBOLT is ultra-fast, highly-integrated, user-friendly and cost-effective, which can greatly reduce computing costs, save time and be free from worry, and help your business develop efficiently.

Laboratories
Hospitals
Research institutes
Enterprises

Fast

MegaBOLT accelerates algorithms such as SOAPnuke, Minimap2, BWA, GATK HaplotypeCaller +MuTect2 through multi-stream and highly parallelized computing architecture, which contributes to a massive speedup in data analysis.

Analysis Time Comparison	Classical Algorithm	MegaBOLT
Analysis Time Comparison	Classical Algorithm	MegaBOLT	ZBOLT	ZBOLT Pro
Germline WGS 30X Basic	26.26h	0.69h	0.24h	0.11h
Germline WGS 30X Full	27.20h	0.80h	0.27h	0.12h
Germline WES 100X Basic	4.27h	0.10h	0.04h	0.02h
Germline WES 100X Full	4.37h	0.13h	0.04h	0.02h
Somatic WGS 40X Tumor + 40X Normal	46.44h	3.66h	1.47h	0.42h
Somatic WES 400X Tumer + 400X Normal	7.64h	0.36h	0.14h	0.06h

* Basic: Including the analysis steps from clean data after quality control to variant calls, taking the average time of multiple tasks.

* Full: Includes extra steps of quality control and report generating in addition to the Basic workflow.

* The time consumed is measured by taking the average of completing 6 identical tasks (MegaBOLT)/ 12 (ZBOLT) / 32 (ZBOLT Pro).

Multi-task scheduling system acceleration

Integrated with the self-developed multi-task scheduling system, MegaBOLT supports simultaneous multi-task computing on one single server. The computing efficiency is further improved by 38%~52%. When only a single task needs to be analyzed, the single-task mode can be used to enhance performance by 13% to 30%.

Germline

WGS 30X NA12878 DNBSEQ
WES 100X NA12878 DNBSEQ

* The time consumed is measured by taking the average of completing 6 identical tasks.

Precision

01.Precision

After speedup, the analysis precision remains steadfast. MegaBOLT is also configured with a deep learning variant calling module, MegaBOLT-DV, which is optimized by algorithms and specified neural network model training. When MegaBOLT-DV is used in combination with PCR-Free library preparation and MGI DNBSEQ^TM sequencing technologies, the human WGS variant calling precision can reach an excellent SNP 99.9% and INDEL 99%. Even more, MegaBOLT-RC, a new recalibrating module based on machine learning, can improve precision significantly without using a lot of extra computing resource.

02.Deep learning making sequencing more cost-effective

MegaBOLT applies heterogeneous computing to the workflow from FASTQ input of sequencing data to BAM output of alignment result and VCF/gVCF output of variant calling result.
MegaBOLT adopts a multi-stream computing architecture and divide data into smaller granules for higher efficiency. We also further streamlined the whole process and developed a highly parallelized computing architecture, thus realizing highspeed WGS/WES analysis and computing.

Classical Algorithm

MegaBOLT-DV

Integrated

Integrated with the self-developed laboratory information management system ZLIMS, MegaBOLT realizes completely automated sequencing and analysis. It can work together with MGI DNBSEQ™ sequencers or sequencers of other platforms.

Laboratory device management and real-time monitoring
Sample information recording and tracking
Data transmission and management
Analysis task scheduling and processing
Analysis result/report management and visualization

Easy to use

The Web design makes MegaBOLT simple and easy to use. You can just click a couple of buttons to submit a task and check the results on the website. In this case, a bioinformatic technical background is no longer necessary.
Economic

With a prominent speedup in computing and rigid control over hardware prime cost, a MegaBOLT server works like 10 normal computers in total and is without doubt highly cost-effective.

Heterogeneous computing accommodates
high-speed sequence analysis

MegaBOLT applies heterogeneous computing to the workflow from FASTQ input of sequencing data to BAM output of alignment result and VCF/gVCF output of variant calling result.

We also further streamlined the whole process and developed a highly parallelized computing architecture, thus realizing highspeed WGS/WES analysis and computing.

Publication